array(2) { ["lab"]=> string(4) "1688" ["publication"]=> string(5) "15268" } A protocol for Epon-embedding-based correlative super-resolution light and electron microscopy - 付志飞实验室-超分辨光电关联成像 | LabXing

付志飞实验室-超分辨光电关联成像

简介 专注于超分辨光电关联成像技术的开发和应用

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A protocol for Epon-embedding-based correlative super-resolution light and electron microscopy

2019
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Abstract This protocol describes a detailed step-by-step sample preparation procedure for Epon based post-embedding correlative super-resolution light and electron microscopy (SR-CLEM). The newly developed fluorescent protein, mEosEM, is the core in this protocol. The advantage of this method is to simultaneously obtain high-quality LM and EM images from the same ultrathin section of Epon-embedded sample after conventional chemical fixation.