An integrated method for simultaneously determining 10 classes of per-and polyfluoroalkyl substances in one drop of human serum - Liang Yong

Per- and polyfluoroalkyl substances (PFASs) represent a group of synthetic chemicals, and they have quite different physicochemical properties, which result in difficulties of their simultaneous determination in a single injection. A sensitive, reliable, and fully automated method was developed for simultaneously detecting 10 classes of PFASs (total of 43) in human serum using online Turboflow SPE-UHPLC-MS/MS. This method provided high linearity of matrix-matched calibration standards (R > 0.99), excellent method limits of detection (MLODs) (0.013–0.089 ng mL−1), satisfactory matrix spiked recoveries (84.3–109%) and relative standard deviations (RSDs) (intra-day RSDs: 1.3–12.6%, inter-day RSDs: 1.7–13.8%, inter-week RSDs: 1.8–13.5%, inter-month RSDs: 3.1–12.4%), short analysis time (19 min per sample) and small sample amount requirement (25 μL), which were suitable for large-scale epidemiologic …